Lipid peroxide formation in microsomes. Relationship of hydroxylation to lipid peroxide formation

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Lipid Peroxide Formation in Microsomes

or ferricyanide were not able to replace ascorbate and induce lipid peroxide formation. 2. The rate of ascorbate-induced peroxidation is optimum at pH 6-0 whereas the rate of the NADPH system is optimum at pH7-0. Both systems require phosphate for maximum activity. 3. Lipid peroxide formation occurs at the maximum specific rate in very dilute microsome suspensions (0-15mg. of protein/ ml.). 4. ...

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Mechanisms of lipid peroxide formation in animal tissues.

1. Homogenates of rat liver, spleen, heart and kidney form lipid peroxides when incubated in vitro and actively catalyse peroxide formation in emulsions of linoleic acid or linolenic acid. 2. In liver, catalytic activity is distributed throughout the nuclear, mitochondrial and microsomal fractions and is present in the 100000g supernatant. Activity is weak in the nuclear fraction. 3. Dilute (0....

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1969

ISSN: 0306-3283

DOI: 10.1042/bj1130333